Structural Information from Anisotropic Orientational and Reorientational Averaging - Long Range Order in Solution State NMR
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چکیده
NMR spectroscopy is now established as the most effective method for the determination of three-dimensional structure of biomolecules in the solution state (Wüthrich 1986). The success of this technique relies primarily on the measurement of cross-relaxation effects (nOe) providing distance restraints between protons in close proximity in space (<6Å) supplemented by J scalar couplings which contribute local dihedral angle information (Clore & Gronenborn 1997). In combination with simple modeling of covalent and throughspace interactions, these short range constraints allow the accurate reconstruction of the secondary and tertiary molecular geometry of globular proteins.
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